Microsoft Word - PHT120BF

Authors’ address: V. Kondi and Natalia Mitrica, Centre of Haematology, Bucarest (Rumania) In a previous study, we have shown that sera with anti-platelet antibodies from thrombocytopenic patients or those having received multiple blood transfusions – females alloimmunized by pregnancy – are able to induce inhibition of platelet aggregation [2]. The present investigation is designed to study the activity of anti-human anti-lymphocyte horse and rabbit sera by the inhibition platelet aggregation phenomenon. We studied 2 anti-lymphocyte horse sera and 4 anti-lymphocyte rabbit sera. All these sera tested proved to have an inhibitory effect on platelet aggregation by extrinsic ADP. The anti-lymphocyte sera were obtained by immunization with peripheral human lymphocytes. A quantitative evaluation of the anti-platelet antibodies was established. The presence of the anti-platelet antibodies in the anti-lymphocyte sera may be explained by the common antigens belonging to lymphocytes and platelets [1] or by the presence of platelet antigens in the lymphocyte suspension, insuficiently purified. We consider our test to be very sensitive and simple for the investigation of anti-platelet antibodies and, consequently, for platelet antigen research. References Dausset, J.: Actualités en transfusion sanguine (Masson, Paris 1967). Kondi, V. and Mitrica, N.: Rev. franc. Transfus. 1: 99 (1970).